Molecular Biologist I / Plasmid DNA Production
Our client, a world-leading Pharmaceutical Company in Cambridge, MA is currently looking for a Molecular Biologist I to join their expanding team.
Position Details:
Job Title:
Molecular Biologist I / Plasmid DNA Production Duration:
18 months contract, extendable up to 36 months Location:
Cambridge, MA Note:
The client has the right-to-hire you as a permanent employee at any time during or after the end of the contract.
You may participate in the company group medical insurance plan
Job Description:
Bench Scientist BioMedicine Design (BMD) is seeking a highly motivated Biochemist/Molecular Biologist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA.
Qualified candidates will play an essential role in a high-energy DNA production and protein expression research laboratory.
The group supports non-GMP transient protein lead production across the company's diverse biotherapeutic discovery portfolio to enable the research units and their pre-clinical therapeutic in-vitro and in-vivo needs.
The BEP DNA production and protein expression group is looking for a laboratory technician to provide DNA production support for the protein expression and purification activities in BEP.
The candidate is expected to have a strong background in molecular biology techniques and experience with nucleic acid production and analysis.
The successful candidate will grow bacterial cultures and perform large-scale DNA production activities following standardized protocols.
The candidate will be a key contributor to the scientific team responsible for protein expression and purification.
Responsibilities:
Production of plasmid DNA to support transient recombinant protein expression Conducting routine molecular biology techniques including cell transformations, day cultures, bacterial cultures, Gibson assembly, and digestions Maintaining reagent and DNA inventory Operate and maintain automated liquid handlers for plasmid purification, as well as downstream QC applications like NGS and DNA normalizations Conducting routine DNA characterization and quality control testing using techniques such as gel electrophoresis, fragment analysis, nanodrop, endotoxin testing, and preparation of samples for sequencing analysis (Sanger and NGS) Maintaining daily work records in an organized electronic lab notebook.
The candidate should also be proficient in using an online database for inventory management Effectively communicating with team members to meet strict schedules and deadlines
Qualifications:
Bachelor's degree in biological / life sciences (college grads accepted) OR; Associate's degree / Certification with a minimum of 1-2
years of experience OR; High school diploma with a minimum of 2
years of relevant laboratory research schooling Experience with the production of plasmid DNA Experience conducting routine molecular biology techniques Ability to maintain reagent and DNA inventory Experience handling automations preferred Familiarity with NGS 'Nanopore ' preferred Familiarity with Golden Gate or Gibson assembly preferred Previous experience in a CRO role preferred Location:
This is a fully onsite position in Cambridge, MA Recommended Skills Biology Clinical Works Communication Electronic Lab Notebook Gel Electrophoresis Quality Management Apply to this job.
Think you're the perfect candidate? Apply Now Estimated Salary: $20 to $28 per hour based on qualifications.
Position Details:
Job Title:
Molecular Biologist I / Plasmid DNA Production Duration:
18 months contract, extendable up to 36 months Location:
Cambridge, MA Note:
The client has the right-to-hire you as a permanent employee at any time during or after the end of the contract.
You may participate in the company group medical insurance plan
Job Description:
Bench Scientist BioMedicine Design (BMD) is seeking a highly motivated Biochemist/Molecular Biologist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA.
Qualified candidates will play an essential role in a high-energy DNA production and protein expression research laboratory.
The group supports non-GMP transient protein lead production across the company's diverse biotherapeutic discovery portfolio to enable the research units and their pre-clinical therapeutic in-vitro and in-vivo needs.
The BEP DNA production and protein expression group is looking for a laboratory technician to provide DNA production support for the protein expression and purification activities in BEP.
The candidate is expected to have a strong background in molecular biology techniques and experience with nucleic acid production and analysis.
The successful candidate will grow bacterial cultures and perform large-scale DNA production activities following standardized protocols.
The candidate will be a key contributor to the scientific team responsible for protein expression and purification.
Responsibilities:
Production of plasmid DNA to support transient recombinant protein expression Conducting routine molecular biology techniques including cell transformations, day cultures, bacterial cultures, Gibson assembly, and digestions Maintaining reagent and DNA inventory Operate and maintain automated liquid handlers for plasmid purification, as well as downstream QC applications like NGS and DNA normalizations Conducting routine DNA characterization and quality control testing using techniques such as gel electrophoresis, fragment analysis, nanodrop, endotoxin testing, and preparation of samples for sequencing analysis (Sanger and NGS) Maintaining daily work records in an organized electronic lab notebook.
The candidate should also be proficient in using an online database for inventory management Effectively communicating with team members to meet strict schedules and deadlines
Qualifications:
Bachelor's degree in biological / life sciences (college grads accepted) OR; Associate's degree / Certification with a minimum of 1-2
years of experience OR; High school diploma with a minimum of 2
years of relevant laboratory research schooling Experience with the production of plasmid DNA Experience conducting routine molecular biology techniques Ability to maintain reagent and DNA inventory Experience handling automations preferred Familiarity with NGS 'Nanopore ' preferred Familiarity with Golden Gate or Gibson assembly preferred Previous experience in a CRO role preferred Location:
This is a fully onsite position in Cambridge, MA Recommended Skills Biology Clinical Works Communication Electronic Lab Notebook Gel Electrophoresis Quality Management Apply to this job.
Think you're the perfect candidate? Apply Now Estimated Salary: $20 to $28 per hour based on qualifications.
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